NFK Green™ Assay Kits

Biochemical High-Throughput Screening Assays for Inhibition of Tryptophan-Metabolizing Enzymes

Tryptophan metabolism plays an important role in immune modulation and neurodegeneration. Tryptophan-metabolizing enzymes are drug targets for cancer immunotherapy and neurodegenerative disease. We have developed NFK Green™ assays for the high-throughput screening of the tryptophan-metabolizing enzymes indoleamine 2,3-dioxygenase 1 (IDO1), indoleamine 2,3-dioxygenase 2 (IDO2), and tryptophan 2,3-dioxygenase (TDO).

NFK Green™ assay kit No. of datapoints
(384-well format)		 Catalog no.
Human IDO1 (hIDO1) 1,000
10,000		 NFK001-1K
NFK001-10K
Human IDO2 (hIDO2) 1,000
10,000		 NFK002-1K
NFK002-10K
Human TDO (hTDO) 1,000
10,000		 NFK003-1K
NFK003-10K
Mouse IDO1 (mIDO1) 1,000
10,000		 NFK004-1K
NFK004-10K
Mouse TDO (mTDO) 1,000
10,000		 NFK005-1K
NFK005-10K
Cellular 1,000
10,000		 NFK006-1K
NFK006-10K
Schematic representation of NFK Green™ assay. After enzymatic conversion of L-tryptophan into NFK, the reaction is stopped by addition of NFK Green™, which yields a fluorescent complex that can be detected at the indicated wavelengths.

Reference

Seegers et al. (2014) High-throughput fluorescence-based screening assays for tryptophan-catabolizing enzymes. Journal of Biomolecular Screening, 19 (9):1266-1274.

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